多序列比对

在博学生信

蛋白序列多重比较构建进化树

Phylogenetic analyses of NLRs

The polypeptide sequences of various disease resistance genes from Triticum spp. and related genera were aligned using Muscle v5.1 software. The sequences were assembled to form a Maximum-likehood phylogenetic tree tested by bootstrapping (1000 replications) using FastTree v2.1.11 software. Phylogenetic trees were further processed and visualized using FigTree and iTOL.

muscle多重比较

muscle -align sequence.pep -output sequence.pep.muscle

trimal修剪

trimal -in  sequence.pep.muscle -out sequence.pep.muscle.trimal -automated1

FastTree建树

FastTree sequence.pep.muscle.trimal > sequence.pep.muscle.trimal.tree

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